Semen parameters and seminal plasma protein and biochemical profiles of dogs with benign prostatic hyperplasia after botulinum toxin type A intraprostatic injection

Ciência Rural

Tathiana Ferguson Motheo²  Aracélle Elisane Alves²  Giuliano Queiroz Mostachio²  Maricy Apparício²  Alexandre Pinto Ribeiro¹  Fabiana Ferreira De Souza¹  Maria Denise Lopes¹  Wilter Ricardo Russiano Vicente² 

[1] ,Universidade Estadual Paulista (Unesp) Faculdade de Ciências Agrárias e Veterinária (FCAV) Departamento de Reprodução AnimalJaboticabal SP ,Brasil

关键词: botulinum toxin;    prostate;    seminal plasma;    dog;    toxina botulínica;    próstata;    plasma seminal;    cão;   

DOI  :  10.1590/S0103-84782014000600026

来源: SciELO

【 摘 要 】

This study aimed to determine the effects of different concentrations of botulinum toxin type A (BT-A) on semen parameters, and seminal plasma biochemical and protein profiles of dogs with benign prostatic hyperplasia (BPH). Eighteen sexually intact male dogs with BPH were randomly divided in three groups, and received an intraprostatic injection of saline solution (control group - CG), 250UI (GI) or 500UI (GII) of BT-A under transabdominal ultrasound guidance. Semen was collected at baseline, 2, 4 and 8 weeks after treatment. Semen parameters were determined and seminal plasma pH, total protein (TP), total chlorides (TC), calcium (Ca), potassium (K), and sodium (Na) concentrations were assessed. One-dimensional sodium dodecyl sulfatepolyacrilamide gel eletrophoresis (SDS- PAGE) was performed to determine seminal plasma protein profile. Sperm parameters and seminal plasma pH, TP, TC, Ca and K mean values did not change significantly at any time point and among treated groups (P>0.05). The SDS-PAGE analysis of the pooled fractions identified 31 protein bands with molecular weights ranging from 3.9 to 106.2kDA in all treatment groups during the entire evaluation period. Regardless the used dose, intraprostatic BT-A injection do not alter semen parameters and seminal plasma biochemical and protein profiles of dogs with BPH.

【 授权许可】

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