Topical and intraluminal Carolina Rinse Solution in p44/42 and p38 MAP Kinase activation profile in rabbit jejunum after ischemia and reperfusion

Ciência Rural

Luciana Ramos Gaston Brandstetter¹  Eugênio Gonçalves De Araújo¹  Juan Carlos Duque Moreno¹  Maria Clorinda Soares Fioravanti¹  Veridiana Maria Brianezi Dignani De Moura¹  Patrícia De Almeida Machado¹ 

[1] ,Universidade Federal de Goiás (UFG) Escola de Veterinária e Zootecnia Goiânia GO ,Brasil

关键词: ERK 1/2;    intestine;    ischemia;    p38;    reperfusion;    ERK 1/2;    intestino;    isquemia;    p38;    reperfusão;   

DOI  :  10.1590/S0103-84782013005000149

来源: SciELO

【 摘 要 】

The effects of topical and intraluminal Carolina Rinse Solution (CRS) in p44/42 (ERK 1/2) and p38 MAPK activation profile, in rabbit jejunum after ischemia and reperfusion (I/R), were investigated in this study. Fifteen New Zealand rabbits were allocated in three groups: Sham-operated (A), Ischemia and reperfusion (B) and CRS (C). Groups B and C were subjected to one hour of ischemia and two hours of reperfusion. In group C, ten minutes prior to reperfusion, the bowel lumen was filled with CRS, and the segment was immersed in CRS until reperfusion onset. Ischemia and reperfusion stimulated the phosphorylation of the p44/42 MAPK and p38 MAPK pathways in some layers of jejunum. Progressive activation of p44/42 MAPK was chiefly localized in the crypts of Lieberkühn, circular and longitudinal muscle layers, whereas p38 MAPK was prominently activated in myenteric plexus and both muscle layers. The results of this research indicate that the chosen model of topical and intraluminal CRS does not interfere in p44/42 and p38 MAPK activation profile in rabbit jejunum subjected to I/R.

【 授权许可】

CC BY   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License