Effect of coconut water on growth of olive embryos cultured in vitro

Ciência Rural

Rafaeli Aparecida Vieira De Souza²  Francyane Tavares Braga¹  Tesfahun Alemu Setotaw¹  João Vieira Neto¹  Patrícia Helena De Azevedo²  Virgínia Helena De Azevedo²  Geraldo Magela De Almeida Cançado¹ 

[1] ,Universidade Federal de Mato Grosso (UFMT) Faculdade de Agronomia e Medicina Veterinária Cuiabá MT ,Brasil

关键词: Olea europaea;    culture medium;    complex mixture;    tissue culture;    zygotic embryos;    Olea europaea;    meio de cultura;    mistura complexa;    cultura de tecidos;    embriões zigóticos;   

DOI  :  10.1590/S0103-84782013000200016

来源: SciELO

【 摘 要 】

The experiment was carried out to determine the appropriate dose of coconut water as supplement for in vitro cultivation of zygotic embryos from 19 olive genotypes. The isolated embryos of the olive seeds were immersed on culture medium containing 0 (control), 25, 50, and 100mL L-1 of fresh and sterile coconut water and kept for 45 days under controlled environment. The percentage of germination, shoot length, number of roots, number of leaves and number of internodes were measured for all 19 olive genotypes. The ANOVA of the parameters evaluated showed significant genotypes x doses of coconut water interaction for shoot length, number of leaves and number of internodes and the dose of 100mL L-1 produced the best results overall as indicated by the means of measured parameters. However, the study showed the importance of determining the appropriate dose of coconut water for each genotype under consideration as shown by significant genotype x dose of coconut water interaction effect.

【 授权许可】

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