Expression of matrix metalloproteases-2 and -9 in horse hoof laminae after intestinal obstruction, with or without Hydrocortisone treatment

Ciência Rural

Luciane Maria Laskoski¹  Carlos Augusto Araújo Valadão¹  Rosemeri De Oliveira Vasconcelos¹  Danilo Ferrucci¹  Juliete Aparecida Francisco Silva¹  Dagmar Ruth Stach Machado¹  Renata Gebara Sampaio Doria¹  Fabio De Sousa Mendonça¹ 

关键词: laminitis;    metalloprotease;    horse;    zymography;    immunohistochemistry;    laminite;    metaloproteinases;    equinos;    zimografia;    imunoistoquímica;   

DOI  :  10.1590/S0103-84782013000100012

来源: SciELO

【 摘 要 】

Twenty horses were used in the experiment, for composed control group, (Cg) instrumented group, (Ig;without intestinal obstruction), treated group (Tg;submitted to intestinal obstruction and hydrocortisone treatment) and non-treated group (Ntg;submitted to intestinal obstruction without treatment). Immunohistochemistry and zymography techniques were used for researches on MMPs 2 and 9 in horse hoof laminae. There was an increase in the expression of MMP-2 in animals of Tg and Ntg. MMP-9 increased on animals from groups Ntg and Ig, however there was no rise of this MMP on the Tg when compared to the other groups in the immunohistochemistry analysis. Based on the results, it was observed that the intestinal injury caused by enterotomy and intestinal obstruction raise the quantities of MMPs in the hoof laminae.

【 授权许可】

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