Protocol optimization and histological analysis of in vitro plant regeneration of RB92579 and RB93509 sugarcane cultivars

Ciência Rural

Roberson Dibax²  Giovana Bomfim De Alcantara¹  Marília Pereira Machado¹  João Carlos Bespalhok Filho¹  Ricardo Augusto De Oliveira¹ 

[1] ,Universidade Federal da Fronteira Sul (UFFS)Laranjeiras PR ,Brasil

关键词: 2;    4-dichlorophenoxyacetic acid;    organogenesis;    tissue culture;    acclimatization;    Poaceae;    ácido 2;    4-diclorofenoxiacético;    organogênese;    cultura de tecidos;    aclimatização;    Poaceae;   

DOI  :  10.1590/S0103-84782012005000138

来源: SciELO

【 摘 要 】

The objectives of this study were to establish appropriate conditions for obtaining plant regeneration and acclimatization of the 'RB92579' and 'RB93509' sugarcane cultivars and to elucidate the shoots origin through histological analysis. For both cultivars, obtaining shoots showed better results with the culture of explants on a callus induction medium containing 2.0mg L-1 2,4-dichlorophenoxyacetic acid, followed by cultivation on a shoot induction medium containing 0.1mg L-1 kinetin and 0.2mg L-1 benzilaminopurine. The MS medium without growth regulators proved to be appropriate for elongation and rooting of shoots and the use of the composed substrate of vermiculite + MS salts was effective for acclimatization. Histological analysis revealed that the origin of the shoots in both cultivars occurred through indirect organogenesis.

【 授权许可】

CC BY   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License