Purification and characterization of hyaluronic acid from chicken combs

Ciência Rural

Claudia Severo Da Rosa²  Ana Freire Tovar¹  Paulo Mourão¹  Ricardo Pereira¹  Pedro Barreto¹  Luiz Henrique Beirão¹ 

[1] ,Universidade Federal de Santa Maria (UFSM) Departamento de Tecnologia e Ciência dos Alimentos Santa Maria RS ,Brasil

关键词: glycosaminoglycans;    chicken combs;    hexuronic acid;    glicosaminoglicanos;    co-produtos de frangos;    ácido hexurônico;   

DOI  :  10.1590/S0103-84782012005000056

来源: SciELO

【 摘 要 】

Hyaluronic acid (HA) is an important macromolecule in medical and pharmaceutical fields. The umbilical cord and the chicken comb are some of the tissues richest in this polysaccharide. The profit from obtaining HA from the combs of slaughtered animals is particularly attractive. This work aimed to extract, purify, and characterize HA. The glycosaminoglycan concentration in the chicken comb was found to be about 15µg of hexuronic acid mg-1 of dry tissue. Fractionation using ion exchange chromatography and subsequent identification of the fractions by agarose gel electrophoresis showed that HA corresponded to 90% of the total amount of extracted glycosaminoglycans.

【 授权许可】

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