An antithrombin-dependent sulfated polysaccharide isolated from the green alga Caulerpa cupressoides has in vivo anti- and prothrombotic effects

Ciência Rural

José Ariévilo Gurgel Rodrigues²  Ismael Nilo Lino De Queiroz¹  Ana Luíza Gomes Quinderé¹  Bruno Cunha Vairo¹  Paulo Antônio De Souza Mourão¹  Norma Maria Barros Benevides¹ 

[1] ,Rede Nordeste de BiotecnologiaFortaleza CE ,Brasil

关键词: green alga;    sulfated polymers;    biological events;    coagulation system;    thrombosis;    alga verde;    polímeros sulfatados;    eventos biológicos;    sistema de coagulação;    trombose;   

DOI  :  10.1590/S0103-84782011000400014

来源: SciELO

【 摘 要 】

Red algae sulfated polysaccharides (SPs) have been widely described as anticoagulant and antithrombotic agents; however no description of antithrombotic activity regarding green algae SPs has been reported. Caulerpa cupressoides (Chlorophyta) has three different SPs fractions (SP1, SP2 and SP3). We investigated the effects of SP2 on thrombin activity by antithrombin and in an experimental model of venous thrombosis in rats. The inhibition of thrombin assay was evaluated using antithrombin (AT) in the presence of SP2 and the antithrombotic activity was investigated in rats with thromboplastin as the thrombogenic stimulus. The anticoagulant effects of SP2 are suggested be due to the potentiation of thrombin inhibition by antithrombin (IC50 ~ 10.0µg mL-1) and this mechanism of interaction is different when compared to other studied Caulerpa polysaccharides. SP2 exhibited antithrombotic effects at doses of 1.0 and 2.0mg kg-1 body weight, but at higher doses (>2.0mg kg-1 body weight) this polysaccharide revert the antithrombotic property. No hemorrhagic effect (2.0mg kg-1) was observed. As occurs with red algae SPs, these results indicate that green algae SPs are also capable of exhibiting different in vivo properties.

【 授权许可】

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