Antibody responses in mice after immunization with inactivated bovine respiratory syncytial virus using different adjuvants

Ciência Rural

Fernando Rosado Spilki²  Renata Servan De Almeida¹  Clarice Weis Arns¹ 

[1] ,Universidade Feevale Instituto de Ciências da Saúde Laboratório de Microbiologia MolecularNovo Hamburgo RS ,Brasil

关键词: bovine respiratory syncytial virus;    BRSV;    adjuvants;    murine model;    inactivated vaccines;    vírus respiratório sincicial bovino;    BRSV;    adjuvantes;    camundongos;    modelos murinos;    vacinas inativadas;   

DOI  :  10.1590/S0103-84782010005000188

来源: SciELO

【 摘 要 】

Bovine respiratory syncytial virus (BRSV) causes pneumonia in young cattle. Modified-live-virus (MLV) and inactivated vaccines are currently used for the control of clinical effects of BRSV infections in cattle. On the present research, the stimulation of specific anti-BRSV immunoglobulin isotypes was investigated, through the use of different commercially available adjuvants (Water-in-oil emulsion, Quil A, Aluminum-hydroxide) in inbred mice (Balb/C and C57BL/6). BRSV antibodies were measured using an enzyme-linked immunosorbent assay (ELISA) and the results were compared to the antibody levels induced by immunization of animals using live-BRSV-virus. Water-in-oil emulsion and alum- adjuvant preparations induced higher levels of IgG1 immunoglobulins, whereas Quil A favored the production of IgG2 antibodies, this last being a more appropriate response profile for the specific case of BRSV. Not using adjuvants resulted in poor levels of specific antibodies. The isotype profile of specific antibodies obtained varied greatly depending on the adjuvants used. This information may be useful for the formulation of more effective BRSV inactivated vaccines; however, these findings have to be confirmed in cattle.

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