Comparing plastic syringes and disposable columns to extract n-alkanes in forage and concentrate feeds

Ciência Rural

Dimas Estrasulas De Oliveira²  Luis Orlindo Tedeschi¹ 

[1] ,Universidade do Estado de Santa CatarinaLages SC ,Brasil

关键词: analysis;    digestibility;    intake;    hydrocarbons;    n-alkane determination;    análise;    determinação de n-alcanos;    digestibilidade;    consumo;    hidrocarbonetos;   

DOI  :  10.1590/S0103-84782010005000128

来源: SciELO

【 摘 要 】

Saturated aliphatic hydrocarbons (n-alkanes) were extracted from feed, orts, and bovine fecal samples using disposable, plastic 5mL-syringes as an alternative material to disposable columns, which are normally used in the liquid-solid extraction phase of n-alkanes. For both methods, the n-alkane extracts (carbon chain length between 31 and 36 atoms) were identified using gas chromatography. The linear regression between methods were: 1) feces: column Alkane=2.63+0.92×syringeAlkane [r²=0.94, square root of the mean square error (RMSE)=13.7mg kg-1, n=30] from which the intercept and the slope did not simultaneously differ from zero and unity (P>0.05), respectively; 2) feeds: column Alkane=0.36+1.12×syringeAlkane (r²=0.85, RMSE=1.9mg kg-1, n=21) from which the intercept and the slope did not simultaneously differ from zero and unity (P>0.05), respectively; 3) orts: column Alkane=0.49+0.92×syringeAlkane (r²=0.98, RMSE=1.2mg kg-1, n=15) from which the intercept and the slope did not simultaneously differ from zero and unity (P>0.05), respectively. Materials with low concentration of n-alkanes may affect the values obtained in both methods. These results suggested that disposable plastic syringes might be a viable alternative to columns thus, reducing analytical costs.

【 授权许可】

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