Canine ehrlichiosis: clinical, hematological, serological and molecular aspects

Ciência Rural

Andréa Cristina Higa Nakaghi²  Rosangela Zacarias Machado²  Mirela Tinucci Costa¹  Marcos Rogério André²  Cristiane Divan Baldani² 

[1] ,Universidade Estadual de São Paulo Faculdade de Ciências Agrárias e Veterinárias Departamento de Patologia VeterináriaJaboticabal SP ,Brasil

关键词: Ehrlichia canis;    dog;    IFAT;    Dot-ELISA;    nested PCR;    Ehrlichia canis;    cão;    RIFI;    Dot-ELISA;    nested PCR;   

DOI  :  10.1590/S0103-84782008000300027

来源: SciELO

【 摘 要 】

The aim of the present study was to compare the direct detection methods of Ehrlichia canis (blood smears and nested PCR), serological tests (Dot-ELISA and Immunofluorescent Antibody Test - IFAT), and demonstrate the most suitable test for the diagnosis of different stages of infection. Blood samples and clinical data were collected from 30 dogs examined at the Veterinary Teaching Hospital, UNESP, Jaboticabal, SP, Brazil. The clinical signs most frequently observed were apathy, anorexia, pale mucous membrane, fever, lymphadenopathy, splenomegaly, hemorrhages and uveitis. Evaluating the humoral immune response, 63.3% of the sera were IFAT positive, while 70% were Dot-ELISA positive. By nestedPCR 53.3% of the samples were positive. Comparing these techniques it was concluded that serology and nPCR are the most suitable tests to confirm the diagnosis of canine ehrlichiosis, however it should be always treated as a complementary data to clinical and hematological evaluation. Serology has an important role in the subclinical and in the chronic phase, nPCR is recommended in the acute stage, and, especially, to identify the ehrlichia specie.

【 授权许可】

CC BY   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License