Development of Intracanal Formulation Containing Silver Nanoparticles

Brazilian Dental Journal

João Felipe Bonatto Bruniera¹  Yara Teresinha Corrêa Silva-sousa¹  Marilisa Guimarães Lara¹  André Pitondo-silva¹  Andrea Marcia Marcaccini¹  Carlos Eduardo Saraiva Miranda¹ 

关键词: Endodontics;    dental pulp diseases;    nanoparticles;    silver;    antibacterial agents;   

DOI  :  10.1590/0103-6440201302431

来源: SciELO

【 摘 要 】

This study aimed to synthetize, characterize and evaluate the antimicrobial properties of silver nanoparticles to be used in the development of a root intracanal formulation. Silver nanoparticles (AgNPs) were obtained by reduction of silver nitrate with sodium borohydride and characterized by UV-Visible spectrophotometry, scanning electron microscopy (SEM) and dynamic light scattering (DLS). The antimicrobial activity of nanoparticle formulation was evaluated by determinations of the minimum inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) against different bacterial species by the microdilution method, according to recommendations of the Clinical and Laboratory Standards Institute (CLSI). Three potential vehicles, hydroxyethylcellulose, Carbomer and polyethylene glycol were tested as carriers for formulations containing AgNPs. The efficiency of the synthesis method chosen to produce AgNPs was demonstrated by four characterization techniques. The nanoparticles showed antibacterial activity against all species tested. Incorporation of AgNPs into all experimental vehicles produced stable formulations but the one in hydroxyethylcellulose presented better physical proprieties. The results indicate that silver nanoparticles are potential antiseptic agents to be used in root canals and incorporation in adequate vehicles may favor a broader application.

【 授权许可】

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