Immunohistochemical expression of p63, epidermal growth factor receptor (EGFR) and notch-1 in radicular cysts, dentigerous cysts and keratocystic odontogenic tumors

Brazilian Dental Journal

Cláudia Kallás Gonçalves¹  Eduardo Rodrigues Fregnani¹  Jorge Esquiche Leon¹  Yara Teresinha Corrêa Silva-sousa¹  Danyel Elias Da Cruz Perez¹ 

[1] ,Federal University of Pernambuco Dental School Department of Clinical and Preventive Dentistry, Oral Pathology SectionRecife PE ,Brazil

关键词: radicular cyst;    dentigerous cyst;    keratocystic odontogenic tumor;    p63;    EGFR;    notch signaling;   

DOI  :  10.1590/S0103-64402012000400005

来源: SciELO

【 摘 要 】

The aim of this study was to assess the immunohistochemical expression of p63 protein, epidermal growth factor receptor (EGFR) and Notch-1 in the epithelial lining of radicular cysts (RC), dentigerous cysts (DC) and keratocystic odontogenic tumors (KOT). For this study, 35 RC, 22 DC and 17 KOT were used. The clinical and epidemiological data were collected from the patient charts filed in the Oral Pathology Laboratory, University of Ribeirão Preto, Brazil. Immunohistochemical reactions against the p63, EGFR and Notch-1 were performed in 3-µm-thick histological sections. The slides were evaluated according to the following criteria: negative: <5% of positive cells, low expression: 5-50% of positive cells, and high expression: >50% of positive cells. Moreover, the intensity of EGFR and Notch-1 expressions was also evaluated. Fisher's exact test and Spearman's correlation coefficients were used for statistical analysis, considering a significance level of 5%. Almost all cases demonstrated p63, EGFR and Notch-1 expressions. The p63 expression was significantly higher in KOT (p<0.001). Positive correlation between these immunomarkers was observed. These findings suggest the participation of p63, EGFR and Notch-1 in the development, maintenance and integrity of cystic odontogenic epithelial lining, favoring lesion persistence. The high expression of p63 in KOT suggests that it may be related to their more aggressive biological behavior and marked tendency to recurrence.

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