Hypomethylation of tumor suppressor genes in odontogenic myxoma

Brazilian Dental Journal

Paula Rocha Moreira²  Fabiano Pereira Cardoso¹  João Artur Ricieri Brito¹  Aline Carvalho Batista¹  Carolina Cavaliéri Gomes¹  Ricardo Santiago Gomez¹ 

[1] ,Federal University of Minas Gerais Institute of Biological Sciences Department of MorphologyBelo Horizonte MG ,Brazil

关键词: methylation;    tumor suppressor genes;    odontogenic myxoma;    dental pulp;   

DOI  :  10.1590/S0103-64402011000500013

来源: SciELO

【 摘 要 】

Odontogenic myxoma (OM) is an ectomesenchymal benign odontogenic tumor characterized by spindle or stellate-shaped cells embedded in an abundant myxoid or mucoid extracellular matrix. DNA methylation is characterized by the addition of methyl groups in cytosines within CpG islands in the promoter gene. DNA methylation can decrease the expression of tumor suppressor genes and contribute to the development of neoplastic lesions. The aim of study was to evaluate the methylation pattern of the tumor suppressor genes P16 (CDKN2A), P21 (CDKN1A), P27 (CDKN1B), P53 (TP53) and RB1 in OM and dental pulp. Methylation was evaluated using methylation-specific polymerase chain reaction (PCR). The transcription was studied in some cases by using reverse transcription quantitative PCR. A higher frequency of unmethylated P27, P53, and RB1 samples was observed in the OM when compared with the dental pulp. OM expressed mRNA of all the genes evaluated. Considering all the samples together, the expression of Rb was higher in the unmethylated samples compared with the partially methylated samples. This investigation revealed hypomethylation of the genes P27, P53, and RB1 in OM. In addition, methylation of tumor suppressor genes was found to be an usual event in normal dental pulp.

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