Effects of a novel calcium aluminate cement on the early events of the progression of osteogenic cell cultures

Brazilian Dental Journal

Larissa Moreira Spinola De Castro-raucci²  Ivone Regina De Oliveira¹  Lucas Novaes Teixeira²  Adalberto Luiz Rosa²  Paulo Tambasco De Oliveira²  Marcos Jacobovitz¹ 

[1] ,University of São Paulo Ribeirão Preto Dental School Ribeirão Preto SP ,Brazil

关键词: calcium aluminate cement;    mineral trioxide aggregate;    cell culture;    osteoblast;    alkaline phosphatase;   

DOI  :  10.1590/S0103-64402011000200002

来源: SciELO

【 摘 要 】

The present study evaluated the progression of osteogenic cell cultures exposed to a novel calcium aluminate cement (CAC+) in comparison with the gold standard mineral trioxide aggregate (MTA). Cells were enzimatically isolated from newborn rat calvarial bone, plated on glass coverslips containing either CAC+ or a control MTA samples in the center, and grown under standard osteogenic conditions. Over the 10-day culture period, roundening of sample edges was clearly noticed only for MTA group. Although both cements supported osteogenic cell adhesion, spreading, and proliferation, CAC+-exposed cultures showed significantly higher values in terms of total cell number at days 3 and 7, and total protein content and alkaline phosphatase activity at day 10. The present in vitro results indicate that the exposure to CAC+ supports a higher differentiation of osteogenic cells compared with the ones exposed to MTA. Further experimental studies should consider CAC+ as a potential alternative to MTA when the repair of mineralized tissues is one of the desired outcomes in endodontic therapy.

【 授权许可】

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