Collagen analysis in human tooth germ papillae

Brazilian Dental Journal

Ivete Jorge Abrahão²  Manoela Domingues Martins¹  Emílio Katayama²  João Humberto Antoniazzi²  Angelo Segmentilli¹  Márcia Martins Marques² 

[1] ,University of São Paulo School of Dentistry Department of EndodonticsSão Paulo SP ,Brazil

关键词: dental papillae;    extracellular matrix;    odontogenesis;    type I collagen;    type III collagen;   

DOI  :  10.1590/S0103-64402006000300006

来源: SciELO

【 摘 要 】

The extracellular matrix (ECM) performs a very important role in growth regulation and tissue differentiation and organization. In view of this, the purpose of this study was to analyze the collagen, the major organic component of dental pulp ECM, in papillae of human tooth germs in different developmental phases. The maxillas and mandibles of 9 human fetuses ranging from 10 to 22 weeks of intrauterine life were removed and 16 tooth germs (1 in the cap stage, 8 in the early bell stage and 7 in the late bell stage) were obtained. The pieces were processed for histological analysis and stained with hematoxylin-eosin, Masson's Trichrome and picrosirius staining technique. Both types of collagen in the dental papilla were only detected by the picrosirius staining technique under polarized light microscopy. Type III collagen was detected in all specimens. Type I collagen was present in focal areas of the dental papilla only in some specimens. In conclusion, the findings of this study showed that type III collagen is a regular component of the papillae of human tooth germs whereas type I collagen is present in a significantly lesser amount.

【 授权许可】

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