Chemical Characterization and Optimization of the Extraction Process of Bioactive Compounds from Propolis Produced by Selected Bees Apis mellifera

Journal of the Brazilian Chemical Society

Tatiane Luiza Cadorin Oldoni¹  Sheila C. Oliveira¹  Suelen Andolfatto¹  Mariéli Karling¹  Matheus A. Calegari¹  Ricardo Y. Sado¹  Fabiana M. C. Maia¹  Severino M. Alencar¹  Vanderlei A. Lima¹ 

关键词: factorial design;    chemical characterization;    phenolic compounds;   

DOI  :  10.5935/0103-5053.20150186

来源: SciELO

【 摘 要 】

The extraction process of bioactive compounds from propolis produced by selected bees was optimized using a 2³ factorial design planning to evaluate the effect of ethanol concentration, time and temperature on the extraction of total phenolic compounds and antioxidant activity. Analysis of total flavonoids, antioxidant activity and chemical characterization performed by high-performance liquid chromatography with photodiode array (HPLC-PDA) detection were carried out in optimal conditions of extraction. Optimizing results suggest that the best condition for extraction of bioactive compounds was obtained in higher levels: time (45 min), temperature (70 °C) and concentration of ethanol (80%). The extract evaluated in optimal conditions presented good antioxidant activity by the radical scavenging 2,2’-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) and ferric reducing antioxidant power (FRAP) methods and chromatographic analysis identified phenolic acids: para-coumaric, ferulic and caffeic, indicating that this material is a potential source of bioactive compounds.

【 授权许可】

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