Flow Injection Analysis System for Screening Organophosphorus Pesticides by their Inhibitory Effect on the Enzyme Acethylcholinesterase

Journal of the Brazilian Chemical Society

Marcos P. Silva¹  Matthieu Tubino¹  Tereza C. R. Elsholz¹  Olaf Elsholz¹  Sabir Khan¹  Marta M. D. C. Vila¹ 

关键词: pesticides;    organophosphorus;    acetylcholinesterase;    biosensor;   

DOI  :  10.5935/0103-5053.20150301

来源: SciELO

【 摘 要 】

A flow-injection spectrophotometric procedure was developed for screening organophosphorus pesticides. The method is based on the inhibition of acetylcholinesterase immobilized on controlled porous glass beads with acetylcholine chloride as the substrate. Methyl parathion, chlorpyrifos, malathion and dichlorvos have been tested. The analytical peak height for a given acetylcholine chloride concentration correlates linearly with the logarithmic concentration of the pesticides between 1.0 × 10-3 mol L-1 and 1.0 × 10-6 mol L-1. If bromine water is added to the pesticide solution, a dramatic increase is observed in the analytical signal, and correlations with the logarithm of the concentrations are observed from 1.0 × 10-3 to 1.0 × 10-10 mol L-1.

【 授权许可】

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