Matrix solid-phase dispersion versus ultrasound assisted extraction with solid-phase extraction in the HPLC analysis of furanocoumarins from fruits of Archangelica officinalis Hoffm

Journal of the Brazilian Chemical Society

Anna Oniszczuk²  Krystyna Skalicka-woźniak¹  Tomasz Oniszczuk¹  Monika Waksmundzka-hajnos²  Kaziemirz Głowniak¹ 

[1] ,Medical University in Lublin Department of Inorganic Chemistry Lublin,Poland

关键词: Archangelica officinalis Hoffm.;    furanocoumarins;    extraction techniques;    high performance liquid chromatography;   

DOI  :  10.5935/0103-5053.20140093

来源: SciELO

【 摘 要 】

In this study an efficient matrix solid-phase dispersion (MSPD) method for the simultaneous HPLC analysis of furanocoumarins from fruits of Archangelica officinalis Hoffm. was performed. Herbal samples were prepared by an optimized MSPD procedure using C18 as sorbent. The analysis was performed by high performance liquid chromatography with diode array detector (HPLC-DAD). The efficiency of the MSPD method was also compared with ultrasound assisted extraction with solid-phase extraction (USAE with SPE). The MSPD extracted furanocoumarins (isoimperatorin, imperatorin, bergapten, isopimpinellin, xanthotoxin, umbelliferone and xanthotoxol) from Archangelica officinalis with satisfactory recoveries ranging from 91.43% to 96.07% and relative standard deviations lower than 4.34%. The detection limit of various furanocoumarins was found to be in the range of 0.37 mg mL-1 for xanthotoxol to 10.82 mg mL-1 for imperatorin. The results presented in the paper reveal that MSPD is efficient, fast, simple and easy to perform method suitable for the isolation of furanocoumarins from herbs.

【 授权许可】

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