Ehrlich and sarcoma 180 tumour characterisation and early detection by ¹H NMR-based metabonomics of mice serum

Journal of the Brazilian Chemical Society

Caroline W. P. Da S. Grandizoli²  Luise Z. B. Carrenho¹  Thais M. G. De Francisco¹  Arquimedes P. De Santana Filho¹  Guilherme L. Sassaki¹  Fabio Simonelli²  Noemi Nagata²  Maria R. O. Kreuger¹  Francinete R. Campos¹  Andersson Barison² 

[1] ,Paraná Federal University Department of Chemistry NMR CenterCuritiba PR ,Brazil

关键词: cancer;    biomarker;    NMR;    metabonomics;    chemometrics;   

DOI  :  10.5935/0103-5053.20140045

来源: SciELO

【 摘 要 】

The success of cancer treatment is directly related to early detection before symptoms emerge, although nowadays few cancers can be detected early. In this sense, ¹H nuclear magnetic resonance (¹H NMR)-based metabonomics was used to identify metabolic changes in biofluid as a consequence of tumours growing in mice. Through partial least squares discriminant analysis (PLS-DA) analysis of ¹H NMR spectra from serum samples it was possible to diagnose Ehrlich ascites and Sarcoma 180 tumours five and ten days after cell inoculation, respectively. Lipids, lipoproteins and lactate were the main biomarkers at onset as well as in the progress of carcinogenic process. Thus, NMR-based metabonomics can be a valuable tool to study the effects of tumour establishment on the chemical composition of biofluids.

【 授权许可】

CC BY   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License