Method validation for simultaneous determination of cholesterol and cholesterol oxides in milk by RP-HPLC-DAD

Journal of the Brazilian Chemical Society

Luciana C. Bauer²  Débora De A. Santana¹  Marianne Dos S. Macedo¹  Alexandre G. Torres¹  Nilson E. De Souza¹  Julliana I. Simionato² 

[1] ,University of Southwest Bahia Instrumental Studies Department Program in Food Engineering and BasicItapetinga BA ,Brazil

关键词: direct saponification;    7-ketocholesterol;    25-hydroxycholesterol;    liquid chromatography;    dairy fat;   

DOI  :  10.5935/0103-5053.20130283

来源: SciELO

【 摘 要 】

The aim of the present work was to develop and validate a reversed-phase highperformance liquid chromatography with diode array detection (RP-HPLC-DAD) method for determination of cholesterol and cholesterol oxides extracted from milk samples. The method consisted of direct samples saponification and analytes extraction, followed by their determination using RP-HPLC-DAD. Suitable analytical selectivity, linearity and range of application, intra-assay and intermediary precision, recovery, detection and quantification limits (LOD and LOQ) were achieved and, the results showed that the developed RP-HPLC-DAD method can be applied to routine determination of cholesterol and cholesterol oxides in milk samples.

【 授权许可】

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