Assessing stir bar sorptive extraction and microextraction by packed sorbent for determination of selective serotonin reuptake inhibitor antidepressants in plasma sample by non-aqueous capillary electrophoresis

Journal of the Brazilian Chemical Society

Ana Paula F. Catai²  Flávio P. Picheli¹  Emanuel Carrilho¹  Maria Eugênia C. Queiroz² 

[1] ,Universidade de São Paulo Faculdade de Filosofia Ciências e Letras de Ribeirão Preto Departamento de QuímicaRibeirão Preto SP ,Brazil

关键词: antidepressant;    microextraction by packed sorbent;    non-aqueous capillary electrophoresis;    plasma sample;    stir bar sorptive extraction;   

DOI  :  10.5935/0103-5053.20130208

来源: SciELO

【 摘 要 】

Two approaches based on partition equilibrium, stir bar sorptive extraction (SBSE) and microextraction by packed sorbent (MEPS) were evaluated for determination of selective serotonin reuptake inhibitor antidepressants (SSRI): fluoxetine, sertraline, citalopram and paroxetine in plasma sample by non-aqueous capillary electrophoresis (NACE) with spectrophotometric detection. The SBSE/NACE and MEPS/NACE methods showed linear range from the LOQ up to 500 ng mL-1. LOQ values ranged from 10 to 30 ng mL(-1)1 in both techniques, depending on the compound. The inter-assay precision of the methods presented coefficient of variation lower than 12%. According to the analytical validation results, the methods are adequate for SSRI antidepressant analyses at therapeutic levels, consequently, amenable to therapeutic drug monitoring. These methods reduced the volumes of biological samples, the use of organic solvents and the number of steps involved in sample preparation.

【 授权许可】

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