Microwave-assisted extraction combined with HPLC-MS/MS for diagnosis of fungal contamination in building materials

Journal of the Brazilian Chemical Society

Daria Horbik¹  Aldona Łowińska-kluge¹  Zbigniew Górski¹  Ewa Stanisz¹  Agnieszka Zgoła-grześkowiak¹ 

[1] ,Poznan University of Technology Institute of Chemistry and Technical Electrochemistry Institute of Structural Engineering Poznań,Poland

关键词: ergosterol;    building material;    microwave extraction;    bioluminescence imaging;   

DOI  :  10.5935/0103-5053.20130191

来源: SciELO

【 摘 要 】

Ergosterol was used as indicator of fungal contamination to assess mould presence on the building materials. Microwave-assisted saponification followed by pentane extraction was used in the sample preparation step. Samples of brick, plaster, concrete and granite were analyzed by high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). Ergosterol concentrations in laboratory infected samples ranged from 6 to 16 ng g-1, while in samples of naturally occurring materials ranged from 66 to 3020 ng g-1. Limits of detection and of quantitation were 1.7 and 5.5 ng g-1, respectively. Recovery and matrix effect were tested using samples spiked at 100 ng g-1. Mean recovery and mean matrix effect were 65.9 and 111.6%, respectively. Microscopic observations and bioluminescence measurements were carried out for the infected samples to make additional confirmation of fungi presence. The ergosterol concentration determined by HPLC-MS/MS in building materials proved to be a suitable indicator to estimate fungal contamination. The developed method was considerably faster for this aim than bioluminescence measurements and traditionally used culture methods.

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