Identification of lipid components in the abdominal muscle of fall-caught Crangon crangon from a coastal area of the Baltic Sea

Journal of the Brazilian Chemical Society

Adriana Mika²  Edward Skorkowski¹  Piotr Stepnowski²  Marek Gołębiowski² 

[1] ,University of Gdansk Faculty of Chemistry Gdansk,Poland

关键词: Crangon crangon;    lipid components;    HPLC-LLSD;    GC-MS;    MALDI-TOF;   

DOI  :  10.5935/0103-5053.20130061

来源: SciELO

【 摘 要 】

The main aim of this work was to determine the principal lipid classes and compositions of fatty acids (FFAs) in triacylglycerols (TAGs) and phospholipids in adult shrimps Crangon crangon from coastal areas of the Gulf of Gdansk (Baltic Sea, Poland). High performance liquid chromatography with a laser light-scattering detector (HPLC-LLSD) was used to separate the lipid classes. The FFA composition was determined with gas chromatography-mass spectrometry (GC-MS) and matrix-assisted laser-desorption time-of-flight mass spectrometry (MALDI-TOF-MS). The following lipid classes were separated using HPLC-LLSD: TAGs, FFAs, sterols (ST) and polar lipids (PL). The HPLC-LLSD analysis showed the dominance of sterols, which were missing from MALDI-TOF-MS, sterols made up 2.5 mg g-1 of the total lipids on the basis of this analysis. TAGs and FFAs were the next most abundant lipid classes, accounting 1.9 mg g-1 and 2.0 mg g-1 of the total lipids, respectively.

【 授权许可】

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