Simultaneous separation of ciprofloxacin, norfloxacin and ofloxacin by micellar electrokinetic chromatography

Journal of the Brazilian Chemical Society

Gabriel Hancu¹  Aura Rusu¹  Brigitta Simon¹  Georgiana Boia¹  Árpád Gyéresi¹ 

[1] ,University of Medicine and Pharmacy Faculty of Pharmacy Department of Pharmaceutical ChemistryTârgu Mureş,Romania

关键词: fluoroquinolones;    micellar electrokinetic chromatography;    ciprofloxacin;    norfloxacin;    ofloxacin;   

DOI  :  10.1590/S0103-50532012005000057

来源: SciELO

【 摘 要 】

This work describes the development of a simple capillary electrophoresis (CE) method for simultaneous determination of the three most frequently used fluoroquinolone derivatives: ciprofloxacin, norfloxacin and ofloxacin. Due to very similar structural and physicochemical characteristics, ciprofloxacin and norfloxacin cannot be separated by the classic method of capillary zone electrophoresis (CZE). Their separations can be solved using micellar electrokinetic chromatography (MEKC), by adding an anionic surfactant to the buffer solution. The efficiency of the separation can be enhanced through the optimization of analytical parameters. A fast reliable method was developed, and the best results were obtained using a buffer solution containing 25 mmol L-1 sodium tetraborate, 100 mmol L-1 sodium dodecyl sulphate and 5% methanol as organic modifier.

【 授权许可】

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