Determination of sildenafil citrate (Viagra®) in various pharmaceutical formulations by flow injection analysis with multiple pulse amperometric detection

Journal of the Brazilian Chemical Society

Antônio Carlos V. Lopes Júnior²  Rita De Cássia Silva Luz¹  Flávio S. Damos¹  Alexandre S. Dos Santos¹  Diego L. Franco¹  Wallans T. Pio Dos Santos² 

[1] ,Universidade Federal dos Vales do Jequitinhonha e Mucuri Departamento de Farmácia Diamantina MG ,Brazil

关键词: sildenafil citrate;    multiple pulse amperometry;    flow injection analysis;   

DOI  :  10.1590/S0103-50532012005000047

来源: SciELO

【 摘 要 】

This work presents a simple, fast and low-cost method for determining sildenafil citrate (SC) in Viagra® and in similar, generic and manipulated pharmaceutical formulations using flow injection analysis (FIA) with multiple pulse amperometric detection. The method consists on the application of three sequential potential pulses as a function of time. SC is detected at 1.6 and 1.9 V by two different irreversible oxidation processes. The third potential pulse (1.0 V) is applied for the regeneration (cleaning) of the surface of the boron-doped electrode. The limit of detection on an order of 10 nmol L-1 was reached with a relative standard deviation of less than 0.2% (n = 10) for 4.5 X 10-5 mol L-1 CS. The analytical frequency was calculated in 86 injections per hour. The addition recovery studies in all the samples were approximately 100% and the results were validated by chromatographic methods.

【 授权许可】

CC BY   
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