Development of nanoinjector devices for electrospray ionization - tandem mass spectrometry (ESI-MSn)

Journal of the Brazilian Chemical Society

Nilson A. Assunção²  Ernesto S. Nakayasu¹  Sirlei Daffre¹  Emanuel Carrilho¹ 

[1] ,Universidade Federal de São Paulo Departamento de Ciências Exatas e da Terra Diadema SP ,Brazil

关键词: mass spectrometry;    nanoinjector;    microsystems;   

DOI  :  10.1590/S0103-50532012005000036

来源: SciELO

【 摘 要 】

In mass spectrometric (MS) systems with electrospray ionization (ESI), the sample can be analyzed coupled to separation systems (such as liquid chromatography or capillary electrophoresis) or simply by direct infusion. The greatest benefit of the type of injection is the possibility of continuous use of small amounts of samples over a long period of time. This extended analysis time allows a complete study of fragmentation by mass spectrometry, which is critical for structure elucidation of new compounds, or when using an ion trap mass analyzer. The injector filled with the sample is placed at the ESI source inlet creating an electric field suitable for the continuous formation of a spray (solvent and sample) and consequently, the gradual and even release of the sample. For the formation of the spray, is necessary that the injector end is metalized. The formation of a bilayer of titanium and gold provided an excellent attachment of the film, resulting in a nanoinjector for ionization/spray formation in the system for MS. The nanoinjectors showed high repeatability and stability over 100 min by continuous sampling with 10 µL of sample.

【 授权许可】

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