Enzymatic hydrolysis of cassava starch for production of bioethanol with a colombian wild yeast strain

Journal of the Brazilian Chemical Society

Mónica I. Ruiz²  Clara I. Sanchez¹  Rodrigo G. Torrres²  Daniel R. Molina² 

[1] ,Universidad Industrial de Santander Facultad de Ciencias Facultad de Salud Escuela de QuímicaBucaramanga,Colombia

关键词: cassava starch;    enzymatic hydrolysis;    amylases;    bioethanol;   

DOI  :  10.1590/S0103-50532011001200014

来源: SciELO

【 摘 要 】

Enzymatic hydrolysis of cassava starch for producing glucose syrups was evaluated using alpha-amylase from Bacillus licheniformis and glucoamylase from Aspergillus niger. Moreover, an enzyme mixture of α-amylase from Aspergillus kawachi and glucoamylase from Aspergillus niger was tested. Enzyme conditions for starch hydrolysis were optimized by a factorial experimental design (3³×2) using as variables substrate concentration, enzyme/substrate ratio and time reaction. Optimal enzyme reactions with 100 g of starch per L were: α-amylase at pH 5.0, 80 °C and enzyme dosage of 130.5 U g-1 of starch; and glucoamylase, pH 4.5, 70 °C and enzyme dosage of 81.5 U g-1 of starch. Additionally, optimal conditions for the enzymatic mixture were pH 4.5, 46 °C, and enzyme dosage of 16.4 U g-1 of starch. Finally, alcohol production using glucose syrups from enzymatically-hydrolyzed starch was carried out with a wild strain of Candida sp isolated from sugar cane juice, obtaining volumetric ethanol productivities around 1.8-3.2 g L-1h-1.

【 授权许可】

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