Utilization of a novel Ag(III)-luminol chemiluminescence system for determination of d-penicillamine in human urine samples

Journal of the Brazilian Chemical Society

Li Ma²  Mengdi Fan¹  Xiangdong Xu²  Weijun Kang²  Hongmei Shi² 

[1] ,Hebei Medical University School of Public Health Shijiazhuang Hebei ,P. R. China

关键词: Ag(III)-luminol;    chemiluminescence;    d-penicillamine;    kinetic characteristic;   

DOI  :  10.1590/S0103-50532011000800009

来源: SciELO

【 摘 要 】

A novel sensitive chemiluminescence (CL) system ([Ag(HIO6)2]5--luminol reaction system), coupled with a flow injection, is introduced for determination of d-penicillamine. The system is based on the enhancement effect of chemiluminescence reaction by penicillamine in alkaline medium. Under the optimum conditions, the CL intensity is proportional to the concentrations of d-penicillamine with a good linearity. The linear range covers 1.5-150 ng mL-1 and the limit of detection of the method is 0.8 ng mL-1. The relative standard deviation is 0.23% for 11 repeated measurements of 0.06 µg mL-1 d-penicillamine. The method has been successfully applied to the determinations of d-penicillamine in pharmaceutical preparations and urine samples, giving rise to satisfactory results. Based on the chemiluminescent spectra and free radical trapping experiment performed in this work, a possible reaction mechanism for this reaction system is suggested.

【 授权许可】

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