Development and validation of analytical method for sulfonamide residues in eggs by liquid chromatography Tandem Mass Spectrometry based on the Comission Decision 2002/657/EC

Journal of the Brazilian Chemical Society

Márcia N. S De La Cruz²  Renata F Soares²  Anna Sylvia F Marques¹  Francisco Radler De Aquino-neto² 

[1] ,Universidade Federal do Rio de Janeiro Instituto de Química LADETECRio de Janeiro RJ ,Brazil

关键词: sulfonamides;    egg;    SPE;    LC-MS/MS;   

DOI  :  10.1590/S0103-50532011000300007

来源: SciELO

【 摘 要 】

Liquid chromatography- electrospray ionization - tandem mass spectrometry (LC-ESI-MS/MS) was used to simultaneous determination of ten sulfonamide (SAs) residues (sulfacetamide, sulfathiazole, sulfamerazine, sulfamethazine, sulfamethoxypyridazine, sulfamethizole, sulfachloropyridazine, sulfamethoxazole, sulfadimethoxine and sulfaquinoxaline) in eggs. Samples were extracted with acetonitrile and the supernatants were cleaned up by solid phase extraction. After solvent evaporation and reconstitution, aliquots of the final extracts were injected into a LC-ESI-MS/MS in positive mode for all sulfonamides. The validated method resulted in recoveries ranging between 87 and 116% with relative standard deviations between 8.5 and 27.2%, CCα between 101.0 and 122.1 ng g-1, and CCβ between 114.5 and 138.8 ng g-1. The analytical method, using fortified samples, was validated based on the Commission Decision 2002/657/EC

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