Bioconcentration of Cd and Pb by the river crab Trichodactylus fluviatilis (crustacea: decapoda)

Journal of the Brazilian Chemical Society

Mariana Franchi²  Amauri A Menegário²  Ana L Brossi-garcia²  Graziela C Chagas²  Monizze V Silva²  Antonio C. S Pião¹  José S Govone¹ 

[1] ,Universidade Estadual Paulista Centro de Estudos Ambientais Rio Claro SP ,Brazil

关键词: bioconcentration;    biomonitor;    metallothionein;    cadmium;    lead;   

DOI  :  10.1590/S0103-50532011000200007

来源: SciELO

【 摘 要 】

The bioconcentration of cadmium and lead by the freshwater crab Trichodactylusfluviatilis was evaluated. Thirty animals were exposed to 200 µg L-1 of cadmium and lead for 7, 14 and 21 days. Both metals were determined in gills, hepatopancreas and muscle after dissection and digestion by inductively coupled plasma optical emission spectrometry. Lead was detected only in gills, but without significant difference among different exposure periods. Cadmium was found in all tissues after exposure. Significant differences among cadmium concentrations in animals exposed for different periods suggest an accumulation process, with concentration stabilized after 14 days. Fractionation of free (or labile) cadmium and cadmium protein (possibly metallothionein) (Cd-P) in gills and hepatopancreas were carried out to assess the cadmium transference and storage in the tissues using a solid phase extraction procedure with Saccharomycescerevisae. Fractionation of free (or labile) cadmium and cadmium protein (possibly metallothionein) (Cd-P) in animals exposed to 200 µg L-1 for 21 days in gills and hepatopancreas were carried to assess the cadmium transference and storage in the tissues using a solid phase extraction procedure with Saccharomycescerevisae. In gills, cadmium was found mainly in the free form, while in hepatopancreas the metal was found mainly bound to the protein (Cd-P). It may be inferred that, absorbed through gills, cadmium was transferred and stored in the hepatopancreas.

【 授权许可】

CC BY   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License