Extraction and purification of C-phycocyanin from Spirulina platensis in conventional and integrated aqueous two-phase systems

Journal of the Brazilian Chemical Society

Francine S. Antelo¹  Andréia Anschau¹  Jorge A. V. Costa¹  Susana J. Kalil¹ 

[1] ,Universidade Federal do Rio Grande Departamento de Química Rio Grande RS ,Brazil

关键词: C-phycocyanin;    aqueous two-phase system;    purity;    protein;    polyethylene glycol;   

DOI  :  10.1590/S0103-50532010000500022

来源: SciELO

【 摘 要 】

Crude C-phycocyanin extracts from Spirulina platensis, with and without cell debris, were purified in aqueous two-phase systems ( ATPS) varying the molar mass of the PEG, at pH 6, more promising than at pH 7 . For the assays carried out with each molar mass, with distinct percentages of PEG and salt, the concentrations (mg mL-1), purities (OD 620/OD 280) and C-phycocyanin partition coefficients of each system were determined and compared. The process for the primary purification of intracellular C-phycocyanin with cell debris (integrated), composed of 15% of PEG 1500 and 13% of potassium phosphate and 5% of PEG 4000 and 18% of salt, produced C-phycocyanin concentrations of, respectively, 1.60 and 2.67 mg mL-1 in the top phase. The purities were of 0.73 and 0.79 respectively, values higher than those obtained in the systems without cells.

【 授权许可】

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