Effects of temperature and chromium (III) ion on the structure of bovine β-Lactoglobulin-A

Journal of the Brazilian Chemical Society

Adeleh Divsalar²  Ali Akbar Saboury²  Faizan Ahmad¹  Ali Akbar Moosavi-movahedi² 

[1] ,University of Tehran Institute of Biochemistry and Biophysics Tehran,Iran

关键词: β-lactoglobulin;    temperature;    Cr(III) ions;    tertiary structure;    Tm value;   

DOI  :  10.1590/S0103-50532009001000003

来源: SciELO

【 摘 要 】

Thermodynamic studies of the effect of temperature (27-47 ºC) on the structure of bovine b-lactoglobulin-A (BLG-A) in the absence and presence of Cr(III) containing 50 mmol L-1 sodium chloride have been carried out using UV-Visible absorption spectroscopy, far and near circular dichroism (CD) and fluorescence spectroscopy. The far-UV CD studies do not show any significant change in the secondary structure of the protein at different temperatures in the absence and presence of Cr(III). On the contrary, the near-UV CD studies show change in the tertiary structure of the native BLG on increasing the temperature, indicating the exposure of Tyr residues. Fluorescence spectroscopic studies on the native BLG-A in the absence and presence of Cr(III) represent considerable change in the tertiary structure of the protein due to the increase in temperature. Due to protein stabilization in the presence of Cr(III) ions, the tertiary structure of BLG represents considerable alterations at 37 and 47 ο C that is in agreement with increasing of Tm values at these temperatures.

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