Potential application in biocatalysis of mycelium-bound lipases from Amazonian fungi

Journal of the Brazilian Chemical Society

Sandra P. Zanotto²  Israel P. Romano²  Líliam U. S. Lisboa²  Sergio Duvoisin Jr.²  Mayra K. Martins¹  Fabiana A. Lima²  Soraya F. Silva²  Patrícia M. Albuquerque² 

[1] ,Universidade do Estado do Amazonas Laboratório de Biorgânica Manaus AM ,Brazil

关键词: fungi;    Amazon region;    mycelium-bound lipase;    biocatalyst screening;    biocatalysis;   

DOI  :  10.1590/S0103-50532009000600008

来源: SciELO

【 摘 要 】

In this study, 212 fungi were isolated from Amazon region plants, aiming to obtain mycelium bound-lipase-producing biocatalysts. These isolates were submitted to hydrolytic and synthetic activity assays. When submitted to the tributyrine substrate test, 87% of the isolates showed hydrolytic activity. Of these, 30% showed good growth in lipase inducing liquid media and were submitted to evaluation of synthetic activity in esterification and transesterification reactions in organic solvents. The nine fungi which had the best synthetic activity were evaluated in the (R, S)-2-octanol resolution reaction, in order to verify the enantioselectivity of mycelium-bound lipases. The isolate UEA_115 was the most versatile biocatalyst, showing good performance in esterification reactions (conversion > 90%) and good ability for the resolution of (R, S)-2-octanol (ee s 29%; ee p 99%; c 22%; E > 200). Thus, this study has demonstrated the great potential of the Amazonian fungi as lipase suppliers for biocatalysts.

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