Antioxidant capacity and biological activity of essential oil and methanol extract of Conobea scoparioides (Cham. & Schltdl.) Benth.

Journal of the Brazilian Chemical Society

Monaliza M. Rebelo¹  Joyce Kelly R. Da Silva²  Eloísa Helena A. Andrade¹  José Guilherme S. Maia¹ 

[1] ,Universidade Federal do ParáBelém PA ,Brazil

关键词: Conobea scoparioides;    Scrophulariaceae;    pataqueira;    thymol and thymol methyl ether;    antioxidant capacity;    cytotoxicity;   

DOI  :  10.1590/S0103-50532009000600005

来源: SciELO

【 摘 要 】

The essential oils of the sub-aquatic plant Conobea scoparioides (fresh and dried previously) showed yields of 3.4 and 3.3%, respectively. The main identified constituents were thymol methyl ether (39.6 and 47.7%), thymol (40.0 and 26.4%), α-phellandrene (12.1 and 14.3%) and p-cymene (1.5 and 1.7%), totalizing more than 90% of the oils. The DPPH radical scavenging activity (EC50) of the oils and extract was 46.7 ± 3.6 µg mL-1 in the fresh plant (CsO-f), 56.1 ± 2.4 µg mL-1 in the dried plant (CsO-d), and 23.0 ± 2.2 µg mL-1 in the methanol extract. The extract (CsE-d)value is comparable to BHT (19.8 ± 0.5 µg mL-1) used as antioxidant standard. The mean value of the oils is twice smaller but equally important as antioxidant agent. The mean amount of Total Phenolics (TP, 124.6 ± 8.7 mg GAE per g) and the Trolox Equivalent (TEAC, 144.1 ± 4.9 mg TE per g) of the methanol extract confirmed the significant antioxidant activity of C. scoparioides. Similarly, in the brine shrimp bioassay (Artemia salina) the mean value of the lethal concentration for the oils (LC50, 7.7 ± 0.3 µg mL-1) was ten times higher than the methanol extract (LC50, 77.6 ± 7.1 µg mL-1) showing important biological activity.

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