Headspace solid-phase microextraction procedure for gas-chromatography analysis of toluene in urine

Journal of the Brazilian Chemical Society

Diego C. Netto¹  Rafael M. Reis¹  Camila B. Mendes¹  Paulo C. F. L. Gomes¹  Isarita Martins¹  Maria Elisa P. B. Siqueira¹ 

[1] ,Universidade Federal de Alfenas Laboratório de Análises Toxicológicas Alfenas MG ,Brazil

关键词: urinary toluene;    HS-SPME;    sample preparation;    gas-chromatography;   

DOI  :  10.1590/S0103-50532008000600022

来源: SciELO

【 摘 要 】

Toluene is widely used in industrial and laboratory applications and in many countries is related with social problems of abuse. Unaltered urinary toluene was introduced as a bioindicator of occupational exposure to the solvent, but its analysis presents difficulties due to the low levels of the compound excreted in urine. A gas-chromatography/flame ionization method for toluene in urine is described using headspace solid-phase microextraction and establishing the better conditions for two different extracting phases: polydimethylsiloxane (PDMS) and carboxen-PDMS. The carboxen-PDMS fiber showed lower quantifying limit (12.5 ng mL-1), better extraction efficiency (up to 28.1%) and repeatability (CV < 4.9%) than PDMS coating. The method was applied to analysis of toluene in urine of workers from car repair shops exposed to low solvent levels.

【 授权许可】

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