Hydroxylation of a hederagenin derived saponin by a Xylareaceous fungus found in fruits of Sapindus saponaria

Journal of the Brazilian Chemical Society

Michael Murgu²  Luiz F. Arruda Santos²  Gezimar D. De Souza²  Cristina Daolio²  Bernd Schneider¹  Antônio Gilberto Ferreira²  Edson Rodrigues-filho² 

[1] ,Universidade Federal de São Carlos Departamento de Química São Carlos SP ,Brazil

关键词: Sapindus saponaria;    saponin;    Xylaria;    biotransformation;    hydroxylation;   

DOI  :  10.1590/S0103-50532008000500004

来源: SciELO

【 摘 要 】

During our screening of tropical plants for endophyte microorganisms, a Xylareaceous fungus was found living on the internal part of Sapindus saponaria fruits. The fruits of S. saponaria accumulate great amounts of triterpenoidal and sesquiterpenoidal saponins. The saponin 3-O-(β-D-xylopyranosyl)-(1→3)-α-L-rhamnopyranosyl-(1→2)-α-L-arabinopyranosyl-hederagenin was isolated using chromatographic methods, after alkaline hydrolysis of the crude extract obtained from S. saponaria fruits and added to the culture medium used to grows the fungus. A new saponin was isolated from this experiment by preparative scale HPLC and characterized as a 22α-hydroxy derivative. The structure of this hydroxylated saponin was elucidated based on interpretation of MS/MS data and NMR spectra.

【 授权许可】

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