A biosensor based on immobilization of lactate oxidase in a PB-CTAB film for FIA determination of lactate in beer samples

Journal of the Brazilian Chemical Society

Denise Lowinsohn¹  Mauro Bertotti¹ 

[1] ,Universidade de São Paulo Instituto de Química São Paulo SP ,Brazil

关键词: lactate;    biosensor;    prussian blue modified electrodes;    CTAB;    FIA amperometric detection;    beer;   

DOI  :  10.1590/S0103-50532008000400005

来源: SciELO

【 摘 要 】

An amperometric lactate biosensor with lactate oxidase immobilized into a Prussian Blue (PB) modified electrode was fabricated. The advantage of using cetyltrimethylammonium bromide (CTAB) in the electrodeposition step of PB films onto glassy carbon surfaces was confirmed taking into account both the stability and sensitivity of the measurements. The biosensor was used in the development of a FIA amperometric method for the determination of lactate. Under optimal operating conditions (pH = 6.9, E = -0.1 V), the linear response of the method was extended up to 0.28 µmol L-1 lactate with a limit of detection of 0.84 mmol L-1. The repeatability of the method for injections of a 0.28 mmol L-1 lactate solution was 2.2 % (n = 18). The usefulness of the method was demonstrated by determining lactate in beer samples and the results were in good agreement with those obtained by using a reference spectrophotometric enzyme method.

【 授权许可】

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