Preparation and characterization of carboxyl-group functionalized superparamagnetic nanoparticles and t he potential for bio-applications

Journal of the Brazilian Chemical Society

Zhi Shan²  Wan-shen Yang²  Xu Zhang²  Qian-ming Huang²  Hui Ye¹ 

[1] ,Sichuan Agriculture University Faculty of Science Sichuan PR ,China

关键词: superparamagnetic;    magnetic nanoparticles;    carboxyl-group;    plasmid DNA;   

DOI  :  10.1590/S0103-50532007000700006

来源: SciELO

【 摘 要 】

In this work, a method was developed to prepare monodispersed carboxyl-group functionalized magnetic nanoparticles. Nanosized maghemite (y-Fe2O3, 7.0 ± 1.0 nm) was synthesized using thermal co-precipitation method and subsequently coated with functional groups by one-step suspension copolymerization. The Fourier transform infrared spectroscopy study and thermogravitmetric analyses confirmed the successful functionalization of carboxyl groups on the surface of magnetic nanocrystals. The surface chemistry makes it possible for SPRI (solid phase reversible immobilization)-based DNA purification. Thus the nanoparticles were employed to isolate plasmid DNA from bacterial cell culture and the results demonstrated its applicability in DNA preparation.

【 授权许可】

CC BY   
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