Electron-transfer functionality of synthetic coiled-coil metalloproteins

Journal of the Brazilian Chemical Society

Michael Y. Ogawa¹  Jiufeng Fan¹  Anna Fedorova¹  Jing Hong¹  Olesya A. Kharenko¹  Anna Y. Kornilova¹  Robin C. Lasey¹  Fei Xie¹ 

[1] ,Bowling Green State University Department of Chemistry Center for Photochemical SciencesBowling Green,USA

关键词: de novo design;    metalloprotein;    electron-transfer;    CuI;   

DOI  :  10.1590/S0103-50532006000800006

来源: SciELO

【 摘 要 】

The emerging field of metalloprotein design seeks to prepare artificial proteins whose properties can mimic, enhance, and perhaps improve upon many features found in natural metalloenzymes. This review summarizes our recent efforts to prepare synthetic metalloproteins built from alpha-helical coiled-coils and to incorporate electron-transfer functionality within these systems. We have recently designed a cysteine-containing random-coil peptide which forms a alpha-helical coiled-coil upon binding various metals. The CuI adduct can serve as photoinduced electron-transfer agent to exogenous acceptors and undergoes collisional electron-transfer in the inverted Marcus region to various ruthenium ammine acceptors. It is speculated that this unexpected result might be due to the positioning of the CuI cofactor within the hydrophobic core of the protein which prohibits close approach between the donor and acceptor to slow the high driving force reaction rates below the diffusion limit.

【 授权许可】

CC BY   
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