Development of a method by HPLC to determine LAS and its application in anaerobic reactors

Journal of the Brazilian Chemical Society

Iolanda C. S. Duarte¹  Lorena L. Oliveira¹  Andréa P. Buzzini¹  M. Angela T. Adorno¹  M. Bernadete A. Varesche¹ 

[1] ,Universidade de São Paulo Escola de Engenharia de São Carlos Departamento de Hidráulica e SaneamentoSão Carlos SP ,Brazil

关键词: Linear Alkylbenzene Sulfonate;    High Performance Liquid Chromatography;    validation;   

DOI  :  10.1590/S0103-50532006000700025

来源: SciELO

【 摘 要 】

This work describes the development and validation of a method using High Performance Liquid Chromatography (HPLC) for Linear Alkylbenzene Sulfonate (LAS) determination in sample of wastewater from anaerobic reactors. The applied LAS standard was the dodecylbenzene sulfonic acid-sodium salt, which presents four main peaks in the chromatogram, related to different homologous of the linear alkyl chain. Different chromatographic conditions were tested with C-18, C-12 and C-8 as stationary phases, ultraviolet and fluorescence detectors, mobile phase (MP) compositions and programming time of elution gradient. The best chromatographic condition was obtained with the C-8 column, MP: methanol and of sodium perchlorate (0.075 mol L-1). The validation of this method was made with the calibration of LAS curves using water and synthetic substrate as solvents. The method was validated in order to demonstrate its precision, linearity, limit of detection of each homolog and its instrumental precision.

【 授权许可】

CC BY   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License