A structural conformation study of aquatic humic acid

Journal of the Brazilian Chemical Society

Luiz F. Zara²  André H. Rosa¹  Ilda A. S. Toscano¹  Julio C. Rocha¹ 

[1] ,Universidade Católica de BrasíliaBrasília DF ,Brazil

关键词: aquatic humic acid;    degree of condensation;    UV/Vis spectroscopy;   

DOI  :  10.1590/S0103-50532006000500028

来源: SciELO

【 摘 要 】

In the present work, aquatic humic substances (HS) were extracted by use of adsorbent XAD 8 and the acid humic fraction (AH) was separated throught acidification. After being purified by Hyphan resin and dialyze, the aquatic AH was characterized using Fourier-transform infrared spectroscopy and elemental analysis. The influence of the aquatic HA and electrolyte concentrations, pH and aquatic AH-metal complexation time on the conformation was investigated using UV/Vis spectroscopic studies, employing the equation suggested by Doty and Steiner. The results indicated that the acid humic flexible macromolecule assumes a condensed form at acid and alkaline pH. Other factors favoring condensed conformations are longer metal complexation time ("ageing") and higher aquatic AH and electrolyte concentrations. Thus considering the strong influence of the investigated parameters in the structural conformation of the humic macromolecule, we conclude that studies using UV/Vis spectroscopy to estimate the concentration, aromaticity, humification degree of the aquatic AH and so on, require rigorous control over the experimental conditions employed to provide a correct interpretation of the analytical results.

【 授权许可】

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