Simultaneous determination and classification of riboflavin, thiamine, nicotinamide and pyridoxine in pharmaceutical formulations, by UV-visible spectrophotometry and multivariate analysis

Journal of the Brazilian Chemical Society

Pedro L. López-de-alba¹  Leticia López-martínez¹  Vìctor Cerdá²  Judith Amador-hernández² 

[1] ,Universidad de Guanajuato Instituto de Investigaciones Científicas Guanajuato Gto,México

关键词: vitamins;    thiamine;    riboflavine;    pyridoxine;    nicotinamide;    SIMCA;    PLS;   

DOI  :  10.1590/S0103-50532006000400012

来源: SciELO

【 摘 要 】

Soft Independent Modeling of Class Analogy and Partial Least Squares Regression were used in this work for the identification and quantification of thiamine, riboflavin, nicotinamide and pyridoxine by UV-Vis spectrophotometry, without separation or preconcentration steps in the analytical procedure. For quantitative purposes, the working range established was 1-14 mg L-1 for riboflavin, 2-26 mg L-1 for thiamine, 2-30 mg L-1 for nicotinamide, and 2-22 mg L-1 for pyridoxine. Recovery results higher than 95% were obtained in all cases during the analysis of synthetic and commercial samples. In the screening of each target vitamin, a classification model was built with two classes: (i) with the vitamin of interest, and (ii) without it. The discriminate capability of each classification model was evaluated for learning, independent testing and commercial samples, resulting in satisfactory findings with exception of riboflavin. Thus, a simple and reliable method is proposed for the simultaneous estimation of these compounds.

【 授权许可】

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