Journal of the Brazilian Chemical Society | |
Improvements in steroid screening in doping control with special emphasis to GC-MS analytical conditions and method validation | |
Marlice A. S. Marques1  Henrique M. G. Pereira1  Francisco R. De Aquino Neto1  | |
[1] ,Universidade Federal do Rio de Janeiro Instituto de Química Rio de Janeiro RJ ,Brazil | |
关键词: steroids; anabolic; doping control; gas chromatography; mass spectrometry; | |
DOI : 10.1590/S0103-50532006000200024 | |
来源: SciELO | |
【 摘 要 】
A procedure is described for the simultaneous determination of androgenic substances including steroids and beta2-agonists. The method involves analysis of hydrolized urinary anabolic compounds using liquid-liquid extraction, with subsequent conversion to trimethylsilylether derivatives for the analysis by GC-MS. Pulse split injection 1/10 of the TMS derivatives at 280 °C into the capillary column, initially maintained at 140 °C then programmed to 180 °C at 40 °C min-1, followed by 3 ºC min-1 to 230 ºC and then 40 ºC min-1 to 300 ºC, resulted in good resolution and peak shape for all compounds. The detection limits of most of the steroids were 1 ng mL-1 except for formebolone and trenbolone (25 ng mL-1). When applied to selected urine samples with evidence of bacterial degradation and metabolites from usual medications/vitamins, the method allowed rapid screening for androgens and other substances monitored in routine. The resolution was adequate to evaluate the endogenous steroid profile relevant to doping control and medical applications.
【 授权许可】
CC BY
All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License
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RO202005130105182ZK.pdf | 299KB | download |