Studies on whole cell fluorescence-based screening for epoxide hydrolases and Baeyer-Villiger monoxygenases

Journal of the Brazilian Chemical Society

Beatriz Bicalho²  Lu S. Chen²  Johann Grognux¹  Jean-louis Reymond¹  Anita J. Marsaioli² 

[1] ,Universidade Estadual de Campinas Instituto de Química Campinas SP ,Brazil

关键词: microorganisms;    Baeyer-Villiger monoxygenase;    epoxide hydrolase;   

DOI  :  10.1590/S0103-50532004000600019

来源: SciELO

【 摘 要 】

Biocatalysis reactions were performed on microtiter plates (200 µL) aiming at the utilization of fluorogenic substrates (100 µmol L-1) for rapid whole cell screening for epoxide hydrolases (EHs) and Baeyer-Villiger monoxygenases (BVMOs). A final protocol was achieved for EHs, with 3 new enzymatic sources being detected (Agrobacterium tumefaciens, Pichia stipitis, Trichosporom cutaneum). The fluorogenic assay for BVMO did not work as expected. However, an approach to possible variables involved (aeration; pH) provided the first detection of a BVMO activity in T. cutaneum.

【 授权许可】

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