Rapid quantitative turbidimetric spot test analysis of potassium in blood serum

Journal of the Brazilian Chemical Society

Matthieu Tubino²  Rafael L. De Souza²  Nelci F. Hoehr¹ 

[1] ,Universidade Estadual de Campinas Instituto de Química Campinas SP ,Brazil

关键词: potassium;    serum;    tetraphenylborate;    turbidimetric;    micro analysis;    spot test;   

DOI  :  10.1590/S0103-50532004000500004

来源: SciELO

【 摘 要 】

A spot test quantitative turbidimetric method was developed for the determination of potassium in blood serum. The precipitation of potassium is done in basic medium with the classical analytical reagent sodium tetraphenylborate. The measurement of the turbidity is directly performed in this solution without further dilution using a micro-turbidimetric cell developed in this work. Blood serum was mixed with the buffer and the analytical reagent solution. The turbidity is measured in a spectrophotometer at 700 nm. The calibration curve is a straight line in the working concentration range, presenting a correlation coefficient equal to 0.9998. The analytical results obtained with this method were compared with those obtained through the use of a potassium ion selective electrode. Complete agreement was achieved between the two methods at the 99% confidence level. The observed mean RSD was 3.8%.

【 授权许可】

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