Enantioselective analysis of omeprazole in pharmaceutical formulations by chiral high-performance liquid chromatography and capillary electrophoresis

Journal of the Brazilian Chemical Society

Pierina S. Bonato²  Fernanda O. Paias¹ 

[1] ,Universidade de São Paulo Faculdade de Ciências Farmacêuticas de Ribeirão Preto Ribeirão Preto SP ,Brazil

关键词: omeprazole;    enantiomer;    chiral HPLC;    chiral CE;    pharmaceutical formulation;   

DOI  :  10.1590/S0103-50532004000200025

来源: SciELO

【 摘 要 】

We developed two sensitive and simple assay procedures based on high- performance liquid chromatography and capillary electrophoresis (CE) for the enantioselective analysis of omeprazole in pharmaceutical formulations. Rac-omeprazole and (S)-omeprazole were extracted from commercially available tablets using methanol:NaOH 2.5 mol L-1 (90:10, v/v). Chiral HPLC separation of omeprazole was obtained on a CHIRALPAK AD column using hexane:ethanol (40:60, v/v) as the mobile phase and detection at 302 nm. The resolution of omeprazole enantiomers by CE was carried out using 3% sulfated beta-cyclodextrin in 20 mmol L-1 phosphate buffer, pH 4.0 and detection at 202 nm.

【 授权许可】

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