Ultrasound-assisted extraction of Ca, K and Mg from in vitro citrus culture

Journal of the Brazilian Chemical Society

Sandra C. C. Arruda²  Adriana P. M. Rodriguez²  Marco A. Z. Arruda¹ 

[1] ,Universidade de São Paulo Centro de Energia Nuclear na Agricultura Laboratório de Biotecnologia VegetalPiracicaba SP ,Brazil

关键词: ultrasound extraction;    FAAS;    FAES;    Ca;    K and Mg determination;   

DOI  :  10.1590/S0103-50532003000300023

来源: SciELO

【 摘 要 】

An ultrasound extraction procedure for Ca, K and Mg from in vitro plant cultures is proposed, comparing cultures of different embryogenic levels of Citrus sinensis and Citrus limonia, employing ultrasound energy. Parameters related to metals extraction, such as plant material sampling, acid concentration and sonication time were investigated. For accuracy check, the proposed ultrasound extraction procedure was compared with a microwave-assisted digestion procedure and no differences in the results were verified at 95% of the confidence level. With this simple and accurate extraction procedure, it was possible to determine differences in Ca, K and Mg concentrations during Citrus embryo formation/development and between cultures (embryogenic and non-embryogenic). Finally, the ultrasound extraction method demonstrated to be an excellent alternative for handless sampling and operational costs.

【 授权许可】

CC BY   
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