Acta Cirurgica Brasileira | |
Preconditioning with mono and polyunsaturated fatty acids and low-intensity electrical stimulation. Effects on skin repair in rats | |
Maria Dos Prazeres Carneiro Cardoso1  Andréa De Oliveira Albuquerque1  Virginia Claudia Carneiro Girão1  Margarida Maria De Lima Pompeu1  Cícero Igor Simões Moura Silva1  Orleâncio Gomes Ritardo De Azevedo1  Sergio Botelho Guimarães1  Paulo Roberto Leitão De Vasconcelos1  | |
关键词: Oils; Electric Stimulation Therapy; Wound Healing; Skin; Rats; | |
DOI : 10.1590/S0102-86502015002000004 | |
来源: SciELO | |
【 摘 要 】
PURPOSE: To evaluate the effects of preconditioning with oils mixes containing ω3/ω6/ω9 associated with micro-currents on skin repair in rats. METHODS: One-hundred and eight Wistar rats randomized into G-1, G-2 and G-3 groups were treated with saline (0.9%), mix 1 (corn+soybean oils) and mix 2 (olive+canola+flaxseed oils), respectively, in a single dose (0.01ml/g) by gavage. Next, each group was subdivided into sham and stimulated subgroups. Pulsed-wave microcurrents (0.5 µA, 0.5 Hz) were applied to stimulated subgroups for 20 min. One hour later anesthetized rats were subjected to surgery. A dorsal incision (6 cm long) was carried out and closed with interrupted nylon sutures. Samples (1cm2) were harvested from the mid-portion of the incision on the 7, 14, 21 post-operative (P.O.) days. Variables were analyzed using Mann-Whitney/Dunn tests Significance level was set to 5 % (p<0.05). RESULTS: Micro-currents promoted increase of exudate and reduction of epithelialization on day 7 in G1 rats. Mixes 1/2 reduced vascularization on 7/14th days P.O. Both 1/2 mixes reduced fibrosis on day 14. Preconditioning with mix 1 led to increased expression of NF-kB on the 7th day. CONCLUSION: Preconditioning with microcurrents has pro-inflammatory effects while oil mixes 1 and 2 decrease fibrosis and vascularization in the proliferative phase of cicatrization.
【 授权许可】
CC BY
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