An evaluation of the protective effect of an infusion of chilled glucose solution on thermal injury of the bile ducts caused by radiofrequency ablation of the liver

Acta Cirurgica Brasileira

Marcelo Salim Talasso¹  Valéria Lanzoni¹  Alberto Goldenberg¹  Rodrigo Gobbo Garcia¹  Rita Maria Aparecida Moura-franco¹  Olívia Oléa Cisnero Fernandes¹  Rafael De Souza Mesquita¹  Jorge Roberto Marcante Carlotto¹  Delcio Matos¹  Gaspar De Jesus Lopes-filho¹  Marcelo Moura Linhares¹ 

关键词: Catheter Ablation;    Radio Waves;    Cold Temperature;    Common Bile Duct;    Liver;    Intraoperative Period;    Swine;   

DOI  :  10.1590/S0102-86502015001000005

来源: SciELO

【 摘 要 】

PURPOSE:To evaluate the protective effects of chilling the bile ducts with cold (5°C) 5% glucose solution (GS) during radiofrequency (RF) administration.METHODS:Twenty male pigs (3 mos. old; 25-30 kg) were subjected to RF delivery with chilling (experimental group, N=10) or without chilling (control group, N=10). Half of the animals in each group were euthanized immediately after the operation, and half were euthanized one week later. The following histological variables in relation to the bile ducts were evaluated by a pathologist (blind examiner): degenerative changes to the epithelium; epithelial necrosis; ulceration, regenerative changes of the epithelium; polymorphonuclear neutrophil infiltration; and thermal effects.RESULTS: The experimental group (88 bile ducts examined) showed reduced thermal damage relative to the control group (86 bile ducts examined) as demonstrated by significant differences in the following histopathological parameters: epithelial detachment of biliary epithelium (84.1% vs. 59.3%; p<0.006); elongation/palisade arrangement of nuclei (65.1% vs. 87.5%; p<0.001); pseudo-goblet cells (32.9% vs. 56.8%; p<0.001).CONCLUSION: Infusion of 5% glucose solution (5°C) has a protective effect on bile ducts subjected to heat (95-110°C, 12 min) from radiofrequency thermal ablation device.

【 授权许可】

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