Effects of perioperative hypothermia and reactive oxygen species in the healing of colonic anastomosis in rats

Acta Cirurgica Brasileira

João Carlos Costa De Oliveira¹  Camila Helena De Oliveira¹  Henrique Eduardo De Oliveira¹  Aline Pereira¹  Marcelo Maraschin¹  Armando José D'acâmpora¹ 

关键词: Anastomosis;    Surgical;    Hypothermia;    Oxidative Stress;    Reactive Oxygen Species;    Rats;   

DOI  :  10.1590/S0102-86502014001800008

来源: SciELO

【 摘 要 】

PURPOSE:We evaluated the hypothesis that induced perioperative hypothermia (32 ± 1ºC) affects the redox balance in the tissue of colonic anastomosis in rats by modifying biochemical enzymatic and non-enzymatic markers related to oxidative stress.METHODS:Forty-eight male Wistar rats were randomly divided into eight experimental groups of six animals each and underwent laparotomy, sigmoid section and immediate anastomosis. Four groups were operated under normothermia (36 ± 1ºC), and the other four under hypothermia (32 ± 1ºC). The animals were reoperated on days 3, 7 and 14 postoperatively, and two groups underwent SHAM at 3 days. From the scar tissue samples, the activity of the antioxidant enzymes superoxide dismutase (SOD) and catalase (CAT) was evaluated, and the levels of non-enzymatic markers of oxidative stress, such as reduced glutathione (GSH) and lipid peroxidation, were measured by the thiobarbituric acid reactive substances (TBARS) assay. The means were compared between groups corresponding to each day of sampling and euthanasia.RESULTS:The hypothermic groups showed a significant reduction on the activity of SOD on day 7 postoperatively, on the activity of CAT on days 7 and 14 postoperatively and on the levels of GSH on day 7 postoperatively. The level of lipid peroxidation was increased in the hypothermia group on day 7 postoperatively and decreased on day 14 compared with the normothermic groups.CONCLUSION:Perioperative hypothermia reduced the activity of the antioxidant enzymes catalase and superoxide dismutase, glutathione levels and increased lipid peroxidation in the scar tissue of colonic anastomoses in rats.

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